Single cell RNA-seq of tumor antigen specific CD8 Tissue Resident Memory (Trm) Cells

Purpose: To characterize the tumor-specific TRM cells in LNs, skin, lung and liver in mice with melanoma-associated vitiligo (MAV). scRNAseq was performed to identify populations of CD8 T cells with TRM transcriptional characteristics.Methods: CD8+Thy1.1+ T cells were sorted from DLN, skin lung and liver of MAV mice and scRNA-sequencedResults: Heterogenouse CD8 memory populations with both circulatating and resident phenotypes were identified.Conclusions: Tumor-specific CD8 T cells form TRM responses in tumor-draining LNs.

scRNA-seq of CD8+Thy1.1+ T cells from DLN, skin, lung and liver of MAV mice 45 days post tumor surgery.